Comparative Biochemical Studies on α-Glucosidases

Part III. Trangsglucosidation Action of an α-Glucosidase of Schizosaccharomuces pombe

Abstract

The transglucosidation action of Schizosaccharomyces pombe α-glucosidase has been in-vestigated by paperchromatography.
The enzyme sythesized some oligosaccharides, consisting mainly of isomaltose, together with panose, isomaltotriose, nigerose, kojibiose and maltotriose by transferring glucosyl moiety from maltose. A small amount of isomaltose was also formed even from free glucose.
The transglucosidation mechanism of the enzyme and the process of the further con-verison of the transglucosidation products were examined. The data indicated that the transglucosidation action of Schizosacch. pombe α-glucosidase also was similar to those of mould α-glucosidase.