Studies on the Myrosinase in Mustard Seed
Part I. The Chromatographic Behaviors of the Myrosinase and Some of its Characteristics
1967 年 31 巻 1 号 p. 18-26
詳細
1967 年 31 巻 1 号 p. 18-26
In order to clarify whether the myrosinase containes only thioglucosidase, or thioglu-cosidase and sulfatase, chromatographic behaviors of the myrosinase on cellulose ion exchanger were studied. Myrosinase showed two peaks by the chromatography on TEAE-cellulose at pH 8.5, but the fractionation of the two enzymes was not accomplished. It was concluded that the two peaks was not ascribable to an artifact but the two closely resembled species of myrosinase existing in the sample solution. Chromatographic separa-tion of the two enzymes on DEAE-cellulose resulted in failure. These results confirmed that the myrosinase was a single β-thioglucosidase originally.
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