Abstract
In order to perform hydrocarbon fermentation satisfactorily, it was necessary to get the sample representing the real feature of the broth. However, Corynebacterium hydrocarboclastus S1OB1, which could accumulate a good deal of L-glutamic acid, formed u. coagulated mass of cells like pellet as they grew up, making the broth heterogeneous, To obtain homogeneous broth, addition of some kinds of surfactants was examined. It was made clear that addition of higher alcohol ethers of polyoxyethylene having H. L. B. value of about 15 was effective and emulsified broth could be obtained. Thus time course studies could be performed in hydrocarbon fermentation.
