抄録
Corynebacterium hydrocarboclastus SIOBI or S489B1 can accumulate a good deal of L-glutamate in a thiamine-deficient medium at the expence of hydrocarbon, but can not form L-glutamate in a thiamine-sufficient medium in spite of rapid cell growth, as already re-ported. In order to establish the optimal culture condition for L-glutamate formation, the influence of the following factors was first studied: hydrocarbon concentration, pH control, nitrogen sources, temperature, aeration and supplement of metal ions or amino acids. Then L-glutamate production from a variety of hydrocarbons or petroleum fractions was examined. Sodium oleate was found to stimulate growth remarkably instead of thiamine. Ferrous ion was found to be obligatory for L-glutamate formation and was suggested to have taken part in the earliest step of n-alkane oxidation.
