Abstract
The effects of cultural conditions on inosine production were investigated by the adenine auxotroph of Corynebacterium petrophilum SB 4082. That, the amount of adenine in the medium was very important on the inosine formation, was cleared. The addition of 10mg of adenine and 0.5 g of yeast extract to 100ml of medium was the best for the inosine formation. Ammonium chloride or ammonium sulphate was effective as nitrogen sources. As the carbon sources, n-C10 to n-C16 were utilized for the growth, but the hydrocarbons from n-C12 to n-C16 were the most suitable for the inosine formation. The inosine fermentation began at 24 hrs. after inoculation. The accumulation of inosine attained to the highest level after five days, the amount of which was 1.6g per liter of the culture filtrate.
