Studies on Biosynthesis of Biotin by Microorganisms

Part VII. Some Properties of Avidin-uncombinable Biotin-Vitamer Produced by Bacillus sp

Abstract

A biotinless mutant (K-681-UV-134) accumulated a large amount of desthiobiotin and an unknown biotin-vitamer in the culture medium.
The parent strain (K-681) of this mutant isolated from soil was identified as Bacillus cereus.
The unknown vitamer was accumulated at the early stage of the incubation in com-parison with desthiobiotin.
The unknown vitamer was purified by the paper- and column-chromatographic methods from the culture filtrate. The purified vitamer gave a single spot when spraying with the ninhydrin reagent after paper chromatographing and its RF values in several solvent systems were identical with those of authentic 7-keto-8-aminopelargonic acid.