Studies on the Corrinoids and Porphyrins in Streptomycetes

Part I. Characterization of 5, 6-Dimethylbenzimidazolylcobamide Coenzyme Formed in the Cells of Streptomyces olivaceus

Abstract

A corrinoid, formed predominantly in the cells of Streptomyces olivaceus 605 grown aerobically on a glucose-lactose medium, was identical with 5, 6-dimethylbenzimidazolyl-cobamide coenzyme (DBC coenzyme) by paper chromatography, paper ionophoresis, absorption spectrophotometry, assay of the coenzyme activity in diol dehydratase system and other tests.
Immediately after the maximal growth, the corrinoid was excreted into the cultural filtrate and converted to various unidentified forms of corrinoids.