Physiological Studies on Thiobacilli

Part II. The Metabolism of Colloidal Sulfur by the Cell-free Enzyme System of Thiobacillus thiooxidans

Abstract

Elemental sulfur was metabolized in the colloidal state by cell-free extracts of T. thiooxidans. The activity was found in the soluble fraction (in the supernatant of centrifu-gation at 130, 000×g for 1hr). The reaction products were sulfide and thiosulfate. p-Chloromercuribenzoate, acetate monoiodide and potassium cyanide inhibited the reaction.