1968 Volume 32 Issue 11 Pages 1331-1336
A new preparative method of NAD was provided by the finding that a large amount of NAD accumulated with AMP, ADP and ATP in the culture broth when Brevibacterium ammoniagenes ATCC 6872 was incubated in the medium containing adenine and nicotinic acid or nicotinamide. A large amount of nicotinic acid mononucleotide (NaMN) with a small amount of NAD accumulated when nicotinic acid or nicotinamide was singly added.
NAD was isolated from the culture broth by ion exchange chromatography, and identified by paperchromatography, ultraviolet and infrared spectra, analyses of ribose and phosphate, and reduction by alcohol dehydrogenase from yeast.
An example of the time course of the fermentation for the production of NAD was shown.
The biosynthetic pathway of NAD from nicotinic acid or nicotinamide in the organism was discussed.
The amount of NAD accumulated reached to several mg per ml.
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