Studies on the Lipoprotein Lipases of Microorganisms

Part II. Effects of Culture Conditions on the Production of Lipoprotein Lipases by Pseudomonas sp. M-12-33

Abstract

The production of lipoprotein lipases by Pseudomonas sp. M-12-33 was improved by an increased aeration. Glucose stimulated the growth of the bacterium but reduced the enzyme production in Bouillon medium. Olive oil remarkably increased the production of lipoprotein lipases but the effect was cancelled by calcium carbonate. Stearic and palmitic acids were as effective as olive oil, but capric and caprylic acids inhibited the enzyme production. Urea was proved to be very effective as a nitrogen source for the production of lipoprotein lipases.