The Changes of “Myosin B” during Storage of Rabbit Muscle

Part V. Denaturation of F-Actin Solution

抄録

The irreversibility of the dissociation of “myosin B” stored in 0.6 M KCl at pH 5.7 and 3°C was attributed to the rapid denaturation of F-actin dissociated from “myosin B”
F-Actin was less stable than myosin A, in 0.18_??_0.60 M KCI at pH 5.7 and temperatures between 0-3°C.
The decrease in the ability of F-actin to bind with myosin A was slightly dependent on storage temperature, and there was no apparent relation with the decrease in the solubility.
A hypothetical scheme for F-actin denaturation was proposed.