Studies on Phyto-peroxidase

Part XX. Peptic Hydrolysis of Performic Acid-Oxidized Peroxidase a of Japanese-radish

Abstract

In a continuation of the structural studies on Japanese-radish peroxidase_??_the products resulting from the action of pepsin on performic acid-oxidized apo-peroxidase a have been examined by ion-exchange chromatography on_??_Dowex 50W-X2 column, followed by gel-filtration chromatography on a Sephadex G-25 column and by high voltage paper electro-phoresis. Seven peptides have been isolated in purified forms in yields of 6 to 39 per cent, and their amino acid compositions have been determined.