1969 Volume 33 Issue 12 Pages 1761-1768
Casein micelles destabilized in milk under frozen storage was compared with stable casein micelles before frozen storage by means of analytical ultracentrifugation.
The stable micelles disaggregated with urea-containing buffer showed a single homogeneous peak of _??_0.9S, while a fast sedimenting subpeak, in addition to the major peak of _??_0.9S, appeared in the destabilized micelles. However, any difference was not found between the stable and destabilized caseins when they were analyzed after removal of calcium. It is suggested that a new type of association is formed, possibly through salt linkages, in the casein system destabilized under frozen storage. Sedimentation pattern of calcium paracaseinate phosphate complex in urea-containing buffer suggests that the destabilization of the micelles by rennin does not involve the change of salt linkages.
This article cannot obtain the latest cited-by information.