Agricultural and Biological Chemistry
Online ISSN : 1881-1280
Print ISSN : 0002-1369
ISSN-L : 0002-1369
Studies on the Bacteria Isolated from Wine
Part V Isolation and Properties of the Growth Factor from Tomato Juice for a Bacterium Inducing Malo-lactic Fermentation
Teruo AMACHIHajime YOSHIZUMI
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1969 Volume 33 Issue 2 Pages 139-146

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Abstract
The growth factor for malo-lactic fermentation bacteria was partially purified from tomato juice, and some properties of the factor were examined. The factor was comparatively a low molecule, thermo-stable and insoluble in nonpolar organic solvents. The purified factor fraction showed positive reaction with Molisch-, tetrazolium- and Nessler-reagents, suggesting the existence of sugar and nitrogen as the chemical components. The treatment with cellobiase or cellulase-II resulted in the inactivation of the factor and simultaneously released the sugar constituent composed of glucose from the factor. These results indicated that the glucose residue might exist as the β-1, 4-glucosidic linkage in the growth factor.
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