The Incorporation of Leucine-U-¹⁴C into Ipomeamarone

Abstract

1. It was demonstrated by silica gel thin layer chromatography that leucine-U-¹⁴C was incorporated into furanoterpenes, e. g. ipomeamarone, in sweet potato root tissue infected by Ceratocystis fimbriata.
2. Further proof for ipomeamarone synthesis from leucine-U-¹⁴C was obtained by the constancy of the specific radioactivity of ipomeamarone semicarbazone through repetitive crystallization.
3. The synthetic pathway of ipomeamarone from leucine was found to be connected with the synthetic pathway from acetate at least at some steps.
4. Leucine-U-¹⁴C was incorporated into both saponifiable and non-saponifiable materials in the same way as acetate-2-¹⁴C.