Abstract
A new derivative of NAD (Compound I) synthesized from NAD and p-nitrophenyl phosphate by the enzyme preparation of Proteus mirabilis (IFO 3849), was isolated from the reaction mixture.
After degradation of this compound with snake venom nucleotide pyrophosphatase, Compound III was obtained. 5'-NMN was phosphorylated to Compound IV by the same enzyme preparation of P. mirabilis. By the determination of chemical constituents and the degradation with phosphomonoesterases, Compounds III and IV were identified as nicotinamide riboside 2' (3'), 5'-diphosphate, and Compound I was identified was NADP analog which was formed by phosphorylation at the 2' or 3' position of the nicotinamide ribose moiety, not at the 2' position of adenosine moiety of NAD.
