Abstract
Growth responses of Brevibacterium ammoniagenes ATCC 6872, capable of accumulating purine nucleotides, were investigated by the use of completely defined media.
(1) Casamino acids required for its growth could be replaced by a mixture of L-histidine, L-homoserine, glycine, D, L-alanine and L-lysine. Acompletely defined medium for mucleotide productions was thus established by the use of this mixture.
(2) High levels of phosphate inhibited growth markedly, and this inhibition was overcome by the simultaneous addition 1) of hign levels of Mg2+ and 2) of Mn2+, 3) pantothenate and 4) thiamine. Ca2+ had also a stimulatory effect on the growth. Therefore, a clear growth response to Mn2+ levels and the requirement of the two vitamins for growth emerged only under the conditions of high phophate and magnesium salts. These 4 factors were found entirely the same as factors essential for nucleotide accumulations by Br. ammoniagenes.
