Studies on Histidine Fermentation

Part I. L-Histidine Production by Histidine Analog-resistant Mutants from Several Bacteria

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Mutants resistant to 1, 2, 4-triazolealanine (TRA) or 2-thiazolealanine (TA) were derived from Corynebacterium glutamicum ATCC-13761 by mutagenic treatment with N-methyl-N'-nitro-N-nitrosoguanidine. More than eighty percent of these mutants were found to accumulate a large amount of L-histidine in culture broth. Among these histidine producers, KY-10260 which was selected on TRA-containing agar, was used to investigate the cultural conditions for histidine production. The amount of histidine accumulation reached to a level of 6_??_8mg/ml with a medium containing 15% molasses (as glucose) and 4.5% ammonium sulfate.
According to the similar procedure, some histidine producers were derived from other bacteria, Arthrobacter citreus, Brevibacterium favum, Bacillus megaterium, Bacillus subtilis and Nocardia globerula.