Abstract
Inhibitory conditions of the tryptic hydrolyzing activity of benzoyl-DL-arginine p-nitro-anilide were investigated. Trypsin was inhibited probably according to its absorption on a colloidal dispersion which was formed in the reaction mixture containing zinc chelate of o-phenanthroline, the substrate, and phosphate of the buffer constituent over pH 6. Although zinc showed the same effect as its chelate compound did in the higher conc. of the substrate, the presence of more than 2 moles of o-phenanthroline to 1 mole of zinc prevented the formation of the colloidal dispersion.
It was also found that skeleton and side chains of o-phenanthroline and its derivatives were influential factors to show inhibitory effects on the hydrolase activity of trypsin.
