抄録
Coloring reaction of sake caused by exposure to sun-light was investigated. There is one of the coloring reactions in which deferri-ferrichrysin participate. The reaction was named Reaction I.
Deferri-ferrichrysin, tyrosine, Mn2+ and unknown nitrogenous compound named con-veniently compound X were essential for Reaction I and citric acid was stimulative.
Compound X was purified by using Amberlite IR 120 (H type) column, active carbon column, silicic acid column and alumina column, and crystallized from methanol-water (1:1).
The crystals decomposed at 288_??_290°C. The ultraviolet and infrared spectra of the compound X were essentially identical with those of authentic kynurenic acid.
From these results the compound X was identified as kynurenic acid.
Since kynurenic acid alone did not cause the color development and riboflavin could be substituted for kynurenic acid in Reaction I, kynurenic acid may act as photosensitizer in Reaction I.
