抄録
Brevibacterium ammoniagenes ATCC 6872 was previously reported to accumulate a large amount of UMP from uracil or orotic acid.
Subsequent experiments were perfomed for ribotidation of pyrimidine analogues which were worthy of note as carcinostatic agents, and showed that Br. ammoniagenes ATCC 6872 was able to produce remarkably 6-aza-UMP, 5-fluoro-UMP, 5-hydroxy-UMP, 2-thio-UMP, 4-thio-UMP, UDP and -UTP from the corresponding pyrimidine analogues. The ribotides were isolated by chromatography on Dowex-1 and identified by paper chromatography, UV spectra, analyses of ribose and phosphate, and periodate consumption.
It was also found that 6-aza-UMP accumulated in the medium was converted to 6-aza-uridine by various microorganisms.
