Department of Agricultural Chemistry, The University of Tokyo
Gakuzo TAMURA
Department of Agricultural Chemistry, The University of Tokyo Laboratory of Microbiology, Department of Food Engineering, Yonsei University, Seoul, Korea.
Kei ARIMA
Department of Agricultural Chemistry, The University of Tokyo
The milk-clotting activity of Mucor-rennin (Milk clotting enzyme of Mucor pusillus Lindt) was inhibited by reaction of diazo-l-H-tetrazole accompanied with increase of the value of the absorbance of biazo.histidine at 480 nm. The activity did not remain when the absorbance reached 50% of maximum value. It is concluded from these results that one mole of histidine in 2 moles of histidine contained in the enzyme has a relation to active center.
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