Catheptic and Trypsin-inhibiting Activities in the Nutritional Muscular Dystrophy

Abstract

From 50 days on, after vitamin E deficient diet was given to guinea pigs, typical symptoms of muscular dystrophy were observed. Muscle protein of these animals was fractionated into 3 fractions, sarcoplasmic fraction, Triton X-100 soluble fraction and myofibrillar fraction.
Free catheptic activity (hemoglobin-splitting activity in the sarcoplasmic fraction), bound catheptic activity (the same in the Triton X-100 soluble fraction) and trypsin-inhibiting activity (in the sarcoplasm fraction) were determined in normal and dystrophic muscles.
In dystrophic muscles, great increase in free and bound catheptic activities was observed. The increase of free catheptic activity was greater than that of bound catheptic activity.
Trypsin-inhibiting activity was also increased in dystrophic muscles two- or three-fold. At the same time, decrease in the content of myofibrillar protein in dystrophic muscles was observed.
These results were discussed in view of the degradation of protein in dystrophic muscles.