Abstract
An investigation of succinic acid production from n-paraffin under various culture conditions was carried out with Candida brumptii IFO 0731. Ammonium nitrogen was required for both cell growth and succinic acid production. Favorable culture conditions for succinic acid production were ascertained. The productivity was markedly increased by the additions of CaCO3 and organic nutrients. Under the best condition, the largest quantity of succinic acid production, 23.6mg/ml, was obtained in a 67% yield from super heavy n-paraffin after 8 days cultivation.