Abstract
In order to determine the limiting dispersion glutenin in concentrated guanidine hydrochloride, the behavior of glutenin was observed in different concentrations of guanidine hydrochloride. Increase of intrinsic viscosity and decrease of turbidity were observed with increasing concentrations of guanidine hydrochloride up to 6M and a limiting state was attained above 6M. The molecular weight determination also gave a limiting value above 6M. The values obtained were 850, 000 for one preparation and 640, 000 for another. While the turbidity and viscosity in 6.5M guanidine hydrochloride were constant in the pH region from 4 to 5.5, they increased slightly around pH 6 and decreased toward pH 7. The viscosity of glutenin around pH 7 was lower than that around pH 4, but the same molecular weight was obtained at pH 4.0 and 6.9.
