1973 Volume 37 Issue 11 Pages 2565-2570
Bacillus No. Ku-1 isolated from soil produced and alkaline catalase in alkaline media. The characteristic point of this bacteria was especially good growth in alkaline media. The alkaline catalase in the culture fluid was purified by DEAE-cellulose and Sephadex columns. The enzyme was most active at pH 10.0 and was stable at pH 7.0 to 8.5. The sedimentation constant was about 12.5S. The enzyme was strongly inhibited by NaN3, KCN, FeSO4 and Fe2 (SO4)3. Properties of the enzyme are almost same as those of catalases so far reported except optimum pH for enzyme action and Kat.f. value (4.4×104).
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