An Exopolygalacturonase from a Strain of Acrocylindrium

Abstract

An exopolygalacturonase was partially purified from mycelia_??_ extracts of a strain of Acrocylindrium. The enzyme was most active at pH 4.5 and showed a higher affinity for polygalacturonic acid than for oligogalacturonic acids. The enzyme was found to hydrolyze glycosidic linkages at the non-reducing end of polygalacturonic acid molecules, giving only monogalacturonic acid as the reaction product.