Distribution of ATP-inhibited Ribonuclease in Bacteria

抄録

ATP-inhibited ribonuclease was first found in Bacillus amyloliquefaciens cells and was isolated by the authors previously. The distribution of the ATP-inhibited ribonuclease was surveyed on various bacteria. ATP-inhibited ribonuclease was found in only several species of Bacillus genus, i.e., B. subtilis (i), B. amyloliquefaciens (ii), B. cereus (iii), B. cereus var. mycoides (iv), and B. thuringiensis (v). ATP-inhibited ribonuclease of Bacillus genus was subdivided into two types. The first type of enzyme found in (i) and (ii) cells had optimum pH at 5.7 and was sensitive to 5mM EDTA. The second type of enzyme found in (iii), (iv), and (v) cells had optimum pH at 6.8 and was partially inhibited by 5mM EDTA.