Purification and Properties of Alkaline Proteinase from Aspergillus oryzae

Abstract

Alkaline proteinase was purified from culture extract of a strain of Aspergillus oryzae. The process consists of the Amberlite IRC-50 adsorption, column chromatography on DEAE-cellulose and CM-cellulose and Sephadex G-100 gel filtration. The molecular weight of the enzyme was estimated to be about 23, 000 by a gel filtration method. Alkaline proteinase showed neither carboxypeptidase activity nor aminopeptidase activity, but degraded_??_The enzyme was completely inhibited by diisopropylphosphorofluoridate (10^-2M) or potato inhibitor (250μg/ml).