Induction of Efficient Mutant for Production of Flavin-Adenine Dinucleotide from Sarcina lutea

Abstract

In attempt to obtain an efficient mutant for the production of flavin-adenine dinucleotide (FAD) from flavin mononucleotide (FMN) and adenine, Sarcina lutea IAM 1099 was treated with N-methyl-N'-nitro-N-nitrosoguanidine, and a purine base-requiring and adenosine deaminaseless mutant was obtained as the favorable one. The mutant accumulated more than three fold as much as that by the parent strain.