Abstract
Post-mitochondrial fraction of rat liver was submitted directly onto the ultracentrifugation with newly improved discontinuous sucrose gradient system, which consisted of high concentration of sucrose. By this method, free ribosomes, membrane-bound ribosomes and smooth-membranes were fractionated, and the ratio of amounts of free ribosomes to membranebound ribosomes was calculated. The ratio of polysomes to monosomes could also be determined by application of the same procedure on DOC-treated post-mitochondrial fraction.
In hypophysectomized rat liver, it was revealed that the ratio of free ribosomes to membrane-bound ribosomes was not changed as compared with that in control rat liver, while that of polysomes to monosomes was 56% smaller. Most of large polysomes were degraded into smaller polysomes by the operation.
