Abstract
A particulate enzyme preparation from Phaseolus aureus (mung bean) seedlings catalyzed the synthesis of a water insoluble β-1, 3-glucan from UDP-α-D-glucose (UDPG) at high concentrations (0.4_??_20mM) and an alkaline insoluble β-1, 3 and β-1, 4-mixed glucan from UDPG at a low concentration (8.5μM).
Furthermore, the two kinds of β-glucan synthetases which were investigated with two reaction systems at high and low concentrations of UDPG had different properties in optimal pH, stability of enzyme activity, and metallic ion requirement.
