Agricultural and Biological Chemistry
Online ISSN : 1881-1280
Print ISSN : 0002-1369
ISSN-L : 0002-1369
Production of 3, 4-Dihydroxyphenyl-L-alanine (L-DOPA) by Pseudomonas melanogenum
Hajime YOSHIDAYoshitake TANAKAKiyoshi NAKAYAMA
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1974 Volume 38 Issue 2 Pages 455-462

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Abstract
Pseudomonas melanogenum ATCC 17806 required methionine, cysteine, cystine, cystathionine, homocysteine or homocystine for growth. However, the addition of these amino acids decreased remarkably L-DOPA (3, 4-dihydroxyphenyl-L-alanine) production by the bacterium. L-DOPA production by the bacterium was further affected by the amount of the substrate, the method of its addition and by the addition of antioxidants, as was the case with Vibrio tyrosinaticus.
Under suitable conditions about 8mg/ml of L-DOPA were produced from 8.6mg/ml of L-tyrosine.
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