Screening, Isolation and Some Properties of Mouse Tumor Cell Agglutinin

抄録

Fifteen varieties of cell agglutinins which nonspecifically agglutinate 6 kinds of mouse tumor cells were obtained from the culture broths of 180 actinomycetes strains. One of them, the No. 77 cell agglutinin, was partially purified by ethanol precipitation and by ion exchange and gel filtration chromatography. This agglutinin was thermo-labile and its activity varied with temperature and pH. Cell agglutination by this agglutinin was reversed after a short period at pH 6.0, whereas not at pH 7.4. Addition of histidine, histamine, ATP, ADP, EDTA, Ag⁺, Cd²⁺, Cu²⁺, and Hg²⁺ and heat treatment of the cells at 45°C for 10min inhibited the agglutination at pH 6.0. but not at pH 7.4.