Studies on Glyoxalase Inhibitor Isolation of a New Active Agent, MS-3, from a Mushroom Culture

抄録

Cultured broths were screened by measuring the inhibition of glyoxalse, using 105, 000g supernatant of rat liver homogenates as a crude enzyme. An active agent, MS-3 (C₂₁H₂₄O₇), was isolated from a cultured mushroom. ID₅₀ value of MS-3 against the crude glyoxalase was 12 mcg/ml. MS-3 has low toxicity and inhibited the growth of Yoshida rat sarcoma cells in cell culture. The production, isolation, and properties of MS-3 are described.