Antigenicity and Immunogenicity of Cell Surface Fraction of Ehrlich Ascites Tumor Cells

Abstract

A membrane fraction corresponding to one-tenth of the dry weight of the whole cell was prepared from Ehrlich ascites tumor cells. Such a fraction retained antigenicity in immune adherence inhibition test using antisera taken from mice hyperimmunized to Ehrlich tumors. The antigenicity was impaired by freezing and thawing, and disappeared after such treatment was repeated more than five times, but was stable to heating up to 60°C. The above fraction retained immunogenicity to induce resistance of mice against Ehrlich tumors and retained considerable immunogenicity after drying with acetone-ether.