抄録
The formation of kanamycin (KM) and KM-acetyltransferase (KAT) in washed cell suspensions of a strain of Streptomyces kanamyceticus was induced by 4-O-(6-aminoglycosyl)-2-deoxystreptamine, i.e. 6-AG-DOS, but repressed by 6-0-(3-aminoglycosyl)-DOS and KM, i.e. 3-AG-DOS and 6'-AG-DOS-3''-AG. The “feedback” inhibition of the formation of KM and KAT by KM was relieved by addition of 3-AG in the presence of 6-AG-DOS. Acetylation of 6-AG-DOS was supposed to be prerequisite for binding 3-AG. The activity of KAT in a cell-free extract was inhibited by inorganic phosphate, ADP, phosphoenolpyruvate, oxalacetate and malate. Citrate and oleate were available to the acetylation reaction as a substitute for acetate. Sclerin stimulated both formation and activity of KAT throughout the experiments mentioned above.
