Abstract
The distribution of cysteine desulfhydrase activity was studied with crude extract from various bacteria. The enzyme activity was found in strains belonging to Enterobacteriaceae. A bacterial strain isolated from soil and identified as Aerobacter aerogenes I-3-2 had shown markedly high activity.
Cultural conditions for the formation of the enzyme were studied with this strain. L-Cysteine Or L-cystine, is essential as an inducer for the enzyme formation. Addition of glycerol at 0.1_??_0.2% concentration and CaCl2•2H2O at 0.2% promoted the enzyme formation. Cell extract with high enzyme activity was prepared from cells grown at 30°C for 8 hr in a medium containing 0.2% L-cysteine•HCl•H2O, 0.1% glycerol, 0.2% CaC12••2H2O, 0.3% yeast extract, 0.5% meat extract, 0.5% polypepton and 0, 2% NaCl in tap water with the pH 7.5.
