Abstract
From the inosine producing mutants of Brevibacterium ammoniagenes, KY 13761 was selected as a strain which produced 6-azauridine from 6-azauracil.
The conditions for the conversion were examined and the intermitent feeding of 6-azauracil was found effective for the accumulation.
In order to increase the accumulation, prototrophic revertants were induced from KY 13761 and KY 13021 was selected. By intermitent feeding of 6-azauracil of a final concentration of 6mg per ml, a maximal accumulation, 12.4mg/ml, of 6-azauridine was obtained with KY 13021.