抄録
The changes in mode of the aggregation of Sake yeasts with cellulose by treatments with various reagents which extract the mannan-protein complex or modify ionized groups on the yeast cell surface was studied. The treatments with the disinfectants such as 70% ethanol and 0.1% mercuric hydrochloride had no effect on the aggregation. In the treatment with dilute NaOH, trypsin, papain and ethylendiamine, which have generally been known as the reagents which extract the mannan-protein complex on the cell surface of yeasts, the aggregation character disappeared or lowered, therefore it was supposed that the mannan-protein complex on the cell surface participated with the aggregation character. The modification of phosphate groups the cell surface with uranyl nitrate and carboxyl groups with 1, 2-epoxypropane and methanolic-HCl had no effect on the aggregation. The modification of amino groups on the cell surface with HNO2 and chloramine T eliminated completely the aggregation character. From above results, it was assumed that the amino groups in the protein of cell surface participated in the aggregation of Sake yeasts with cellulose.
