Identification of the Metabolite (M-1) of 2-tert-Butyl-4-(2, 4-dichloro-5-isopropoxyphenyl)-Δ²-1, 3, 4-oxadiazolin-5-one (Oxadiazon) in Rice Plants

Abstract

Out of metabolites of 2-tert-butyl-4-(2, 4-dichloro-5-isopropoxyphenyl)-Δ²-1, 3, 4-oxadiazolin-5-one (oxadiazon) in rice plants one of the unidentified compounds nominated as M-1 was found much in head parts as compared with the parent compound and other metabolites. Identification of M-1 was made by means of thin-layer chromatography, gas-liquid chromatography, mass spectrometry and coincidence by the synthetic compound.
M-1 was identified as 1-(2, 4-dichloro-5-isopropoxyphenyl)-1-methoxycarbonyl-2-trimethyl-acetyl-hydrazine and a pathway of cleavage of oxadiazolin ring of oxidiazon in rice plants was confirmed.