Abstract
Reduced and cyanoethylated glutenin was fractionated into three fractions (F I, F II and F III) by gel filtration on Sephadex G-100 in 0.1M acetic acid. The molecular weight determination was made with these three fractions by sedimentation equilibrium in 6.5M guanidine hydrochloride containing 0.01M acetic acid. The molecular weight obtained was 44, 000 for F II, and 32, 000 for F III. FI showed a distribution of molecular weight due to the aggregation. The avarage molecular weight of F I was 52, 000, being 27, 000 at the meniscus and 98, 000 at the bottom. Thte estimation of molecular weight by SDS-PAGE* gave overestimated values for glutenin polypeptdes, as was already reported for gliadin.1)
