Abstract
Sclerin (SCL) stimulated the production of aminoglycoside antibiotics. Production of kanamycin (KM) was preceded by formation of KM-N-acetyltransferase and initiated by induction of N-acetyl-KM-amidohydrolase. KM-acetyltransferase rapidly developed and suddenly decreased at an early trophophase, whereas N-acetyl-KM-amidohydrolase appeared late and increased gradually. Addition of SCL to the culture initially most enhanced the productivity of KM, inducing both enzymes. Production of ribostamycin (RM) was also preceded by RM-acetyltransferase and optimal period for SCL addition was initial. On the other hand, production of streptomycin (SM) associated with both SM-(streptidino) kinase and alkaline phosphatase through trophophase and idiophase was rather stimulated by SCL added later. SCL induced alkaline phosphatase but not SM-(streptidino) kinase. Thus, a difference has been found in the effect of SCL between regulation of aminoglycoside-modifying (salvaging) enzymes and productivity of antibiotics.
