Folin-phenol Reagent-positive Material Released during Autolysis of Tissue Slices from Rats

Abstract

Acid-soluble Folin-phenol reagent-positive material (FPM) released during autolysis of tissue slices from rats under various dietary conditions was studied. A marked release of FPM was observed during both aerobic and anaerobic incubations of liver slices from protein-depleted rats as well as during an anaerobic incubation of those from normal rats.
This marked release of FPM was proved, by analyzing acid-soluble fractions on Sephadex G-15 column chromatography, to be almost due to accumulation of xanthine and not to increased proteolysis.
The marked release of FPM under aerobiosis in diaphragm from normal rats was also almost due to accumulation of xanthine.