Agricultural and Biological Chemistry
Online ISSN : 1881-1280
Print ISSN : 0002-1369
ISSN-L : 0002-1369
A Method for Preparing Bead Shaped Immobilized Enzyme
Koji KAWASHIMAKeiji UMEDA
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1976 年 40 巻 6 号 p. 1143-1149

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Monomer solution containing enzyme was frozen to a small bead shape by cool solvent and polymerized by Co-60 gamma ray with a dose of 125_??_850 Krad.
Thus, bead shaped immobilized enzymes with various diameters (0.02_??_15mm) were obtained.
The bead had spongy structure and large surface area. Consequently, even large sized bead shaped immobilized enzyme showed high enzymic activity.
Retained activity of bead shaped immobilized invertase was 16_??_73% depending on bead size.
Bead shaped lipase (1mm diameter) showed 13.5_??_42.5% of retained activity depending on monomer combinations.
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© Japan Society for Bioscience, Biotechnology, and Agrochemistry
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