Structure of Hydroxyproline-arabinoside from Tobacco Cells

Abstract

The hydroxyprolyl tri and tetraarabinosides were isolated from a cell wall preparation of cultured tobacco cells by alkali hydrolysis, gel filtration and ion-exchange chromatography. The structures of the two hydroxyproline-arabinosides (Ara₃-Hyp and Ara₄-Hyp) were determined by analysis of controlled Smith-degradation products, and measurements of PMR spectra and optical rotation to be β-L-Araf-(1→2)-β-L-Araf-(1→2)-β-L-Araf-(1→4)-Hyp and β-L-Araf-(1-→3)-β-L-Araf-(1→2)-β-L-Araf-(1→2)-β-L-Araf-(1→4)-Hyp, respectively.