Gas-liquid Chromatographic Determination of Optical Purity of Allethrolone and Allethronyl Chrysanthemate

Abstract

Gas-liquid chromatographic(GLC) method was developed for the determination of the optical purity of allethrolone and allethrolone moiety in allethronyl chrysanthemate. Allethrolone was reacted with N-trifluoroacetyl(TFA)-L-prolyl chloride to give diastereoisomer esters, which were separated from each other on a 10% silicone DC QF-1 column (3mm i. d.×2.25m) and the optical isomers of allethrolone were determined by their peak areas. (+)-Allethronyl (+)-trans-chrysanthemate was hydrolyzed with 0.5 N methanolic potassium hydroxide and the liberated allethrolone was extracted from the hydrolyzate with chloroform and its isomer ratio was determined.