Abstract
Callus was induced from the veins and young petioles of mulberry leaves on modified Linsmaier and Skoog medium with the addition of 3% sucrose, 10-5M of auxin and 10-7M of kinetin. The growth rates of both solid and liquid cultures were nearly the same. The best growth rate was 3_??_4 fold during a 2 week culture period in a jar-fermenter.
To use cultured mulberry cells instead of fresh leaves as the major ingredient in artificial silkworm food, cells needed to be cultured under light to produce a small amount of chlorophyll. IBA was the most effective auxin for obtaining slightly green cells while 2, 4-D was ineffective.
Silkworms fed artificial food which included these cultured mulberry cells, spun good cocoons.
