Synthesis and Separation of N-Tfa-L-leucyl-dipeptide Methyl Esters and N-Tfa-aminoacyl-L-alanyl Amino Acid Methyl Esters by Gas Chromatography

Abstract

A series of Tfa-Leu-Y-Z-OMe and Tfa-X-Ala-Z-OMe, in which X, Y and Z are Gly, Ala, Val, Leu, Pro or Phe, were prepared from the corresponding Cbo-tripeptide methyl esters, respectively, and separation of these tripeptide derivatives by gas chromatography (GLC) was studied. It was found that the relationship between the amino acid composition of the tripeptides and their q_i-values (relative retention values) analogous to the case of the dipeptides could be detectable, although there were some marked exceptions within our limited experiments.