Abstract
Biotin requirements for the growth of a mutant (KY-21-1-25-43-101) of a biotin-producing bacterium (Bacillus sp. KY-21-1-25) was examined and the characteristics of the biotinvitamers produced by the mutant was also examined. Either biotin or dethiobiotin supported the growth of the mutant, but pimelic acid, 7-keto-8-aminopelargonic acid and 7, 8-diamino-pelargonic acid did not support the growth of the mutant. Biotin-vitamers produced from pimelic acid by the mutant were identified as 7-keto-8-aminopelargonic acid and 7, 8-diamino-pelargonic acid. Dethiobiotin was not produced from pimelic acid by the mutant. Intact cells of the mutant had no capacity to synthesize dethiobiotin from 7, 8-diaminopelargonic acid, while the parent strain had a high potency for dethiobiotin synthesis from 7, 8-diamino-pelargonic acid. The mutant had high potency for biotin production from dethiobiotin that was the same as that of the parent strain.
